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Publications
Journal Covers
Publications
Chris Holland's Google Scholar and for the complete paper collection including Endnote Library click here, the password is pretty obvious if you know what I've worked with
2025
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The remarkable toughness (>70 MJ m−3) of silkworm silk is largely attributed to its hierarchically arranged nanofibrilla…r nanostructure. Recreating such tough fibers through artificial spinning is often challenging, in part because degummed, dissolved silk is drastically different to the unspun native feedstock found in the spinning gland. The present work demonstrates a method to dissolve silk without degumming to produce a solution containing undegraded fibroin and sericin. This solution exhibits liquid-liquid phase separation above 10% (wt/wt), a behavior observed in the silk gland but not in degummed silk solutions to date. This partitioning enhances the stability of the undegummed solution, delaying gelation two-fold compared with degummed silk at the same concentration. When spun under identical conditions, undegummed solutions produces fibers 8× stronger and 218× tougher than degummed silk feedstocks. Through ultrasonication, undegummed wet spun fibers are seen to possess hierarchical structure of densely packed ≈20 nm nanofibrils, similar to native silks, although completely absent from fibers wet-spun from degummed silk solutions. This work demonstrates that the preservation of molecular weight, presence of sericin and stimulation of liquid-liquid phase separation underpin a new pathway to recreate a hierarchical fiber with structures akin to native silk.
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2024
Metal Cross-Linked Supramolecular Gel Noodles: Structural Insights and Antibacterial Assessment 2024
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2023
The past, present and future of spider silk textiles 2023
Bloomsbury Encyclopaedia of World Textiles. Volume 10. Textile Futures: Section 1 MAKE
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Historical Silk Biomaterials 2023
Chapter 1 in Silk-Based Biomaterials for Tissue Engineering, Regenerative and Precision Medicine, 2nd Edition (Editors: Subhas C. Kundu, Rui L. Reis) November 1, 2023
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2022
Extensional flow affecting shear viscosity: Experimental evidence and comparison to models 2022
Molecules 2022, 27(2), 551
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2021
2020
2019
Revealing Spider Silk's 3D Nanostructure Through Low Temperature Plasma Etching and Advanced Low-Voltage SEM 2019
Frontiers in Materials 5: Article number 84 25
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2018
Hybrid hydrogels based on polysaccharide gum karaya, poly(vinyl alcohol) and silk fibroin 2018
Materials Research Express
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Differential Scanning Calorimetry of Native Silk Feedstock 2018
Macromol. Biosci. 2018, 1800228
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Macromolecular Biosciences
Extensional flow behaviour and spinnability of native silk 2018
Soft Matter, 2018,14, 8838-8845
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The Biomedical Use of Silk: Past, Present, Future 2018
Advanced Healthcare Materials
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The Energy Requirements for Flow‐Induced Solidification of Silk 2018
Macromol. Biosci. 2018, 1800229
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Changes in Silk Feedstock Rheology during Cocoon Construction: The Role of Calcium and Potassium Ions 2018
Macromol. Biosci. 2018, 1800188
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New perspectives on nano-engineering by secondary electron spectroscopy in the helium ion and scanning electron microscope 2018
MRS Communications
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MRS Communications
The rheological properties of native sericin 2018
Acta Biomaterialia
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Biophotonics of Native Silk Fibrils 2018
Macromolecular Bioscience
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2017
Mapping Nanostructural Variations in Silk by Secondary Electron Hyperspectral Imaging 2017
Advanced Materials.
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Advanced Materials
Analysis of the pressure requirements for silk spinning reveals a pultrusion dominated process 2017
Nature Communications volume 8, Article number: 594
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Silk micrococoons for protein stabilisation and molecular encapsulation 2017
Nature Communications.
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Sericin Promotes Fibroin Silk I Stabilization Across a Phase-Separation 2017
Biomacromolecules.
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Progress and Trends in Artificial Silk Spinning: A Systematic Review 2017
ACS Biomaterials Science and Engineering.
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2016
Thermo-rheological behaviour of native silk feedstocks 2016
European Polymer Journal.
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The Rheology behind Stress-Induced Solidification in Native Silk Feedstocks 2016
International Journal of Molecular Sciences.
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Dry-Spun Silk Produces Native-Like Fibroin Solutions 2016
Biomacromolecules, 2016, 17, 10, 3198-3204
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Environmental effects on the construction and physical properties of Bombyx mori cocoons. 2016
Journal of Materials Science, 1-10.
Native Silk Feedstock as a Model Biopolymer: A Rheological Perspective 2016
Biomacromolecules, 2016,17,8, 2662-2671
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Glass transitions in native silk fibres studied by dynamic mechanical thermal analysis 2016
Biomacromolecules, 2016, 17, 10, 3198-3204
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2015
Unpicking the signal thread of the sector web spider Zygiella x-notata 2015
Interface 12 (113) 20150633.
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Rheological behaviour of native silk feedstocks 2015
Polymer, 67, 28-39
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More than one way to spin a crystallite: multiple trajectories through liquid crystallinity to solid silk 2015
Proceedings of the Royal Society B: Biological Sciences 282, 20150259
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Biomechanical testing and development of silk-based textiles for regenerative medicine and surgery 2015
Biomedical textiles for orthopaedic and surgical applications (Blair, T., ed), pp. 191, Woodhead Publishing.
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The use of spider silk as a biomaterial 2015
In Advances in Silk Science and Technology (Basu, A., ed), pp. 298, Woodhead Publishing.
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Linking naturally and unnaturally spun silks through the forced reeling of Bombyx mori 2015
Acta Biomaterialia 11, 247-255.
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2014
Silk protein aggregation kinetics revealed by Rheo-IR 2014
Acta Biomaterialia, Volume 10, Issue 2, February 2014, Pages 776-784
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Horses and cows might teach us about human knees 2014
Naturwissenschaften 101, 351-354.
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The speed of sound in silk: linking material performance to biological function 2014
Advanced Materials 26, 5179-5183.
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Differential Scanning Fluorimetry provides high throughput data on silk protein transitions. 2014
Scientific Reports volume 4, Article number: 5625 (2014)
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2013
In situ tensile tests of single silk fibres in an environmental scanning electron microscope (ESEM) 2013
Journal of Materials Science, 1-8.
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Forced reeling of Bombyx mori silk: separating behavior and processing conditions 2013
Biomacromolecules 14, 3653-3659.
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2012
Tensile and shear mechanical properties of rotator cuff repair patches 2012
Journal of Shoulder and Elbow Surgery 21, 1168-1176.
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Ballistic impact to access the high-rate behaviour of individual silk fibres 2012
Journal of the Mechanics and Physics of Solids 60, 1710-1721.
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Distinct structural and optical regimes in natural silk spinning 2012
Biopolymers 97, 368-373.
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Comparing the rheology of mulberry and “wild” silkworm spinning dopes 2012
Biopolymers 97, 362-367.
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Direct visualization of shear dependent silk fibrillogenesis 2012
Soft Matter, 2012,8, 2590-2594
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Silk and Synthetic Polymers: Reconciling 100 Degrees of Separation 2012
Adv. Mater., 24: 105-109
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2011
Rheo-attenuated total reflectance infrared spectroscopy: a new tool to study biopolymers 2011
Phys. Chem. Chem. Phys., 2011,13, 3979-3984
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Torn human rotator cuff tendons have reduced collagen thermal properties on differential scanning calorimetry 2011
Journal of Orthopaedic Research 29, 1938-1943.
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Comparing normal and torn rotator cuff tendons using dynamic shear analysis 2011
Journal of Bone and Joint Surgery (Br) 93-B, 942-948.
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A poisonous surprise under the coat of the African crested rat 2011
Proceedings of the Royal Society B: Biological Sciences 279, 675-680.
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There are many more lessons still to be learned from spider silks 2011
Soft Matter 7, 9595-9600.
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2009
Concentration state dependence of the rheological and structural properties of reconstituted silk 2009
Biomacromolecules 10, 2724-2728.
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2008
Biomimetic principles of spider silk for high performance fibres 2008
Chapter 7, pages 74-94 in Biologically Inspired Textiles: Woodhead Publishing, Cambridge, UK
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2007
Natural and unnatural silks 2007
Polymer, Volume 48, Issue 12, 4 June 2007, Pages 3388-3392
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2006
Comparing the rheology of native spider and silkworm spinning dope 2006
Nature Materials volume 5, pages870–874 (2006)
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